Western blotting analysis using Cathepsin L/V/K/H antibody (Cat#62056). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cathepsin L/V/K/H antibody (Cat#62056, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Cathepsin L/V/K/H antibody (Cat#62056). Cathepsin L/V/K/H expression in wild type (WT) and Cathepsin L/V/K/H shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Cathepsin L/V/K/H antibody (Cat#62056, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cathepsin L/V/K/H expression in HeLa cells using Cathepsin L/V/K/H antibody (Cat#62056, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cathepsin L/V/K/H.
Validation of Cathepsin L/V/K/H knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cathepsin L/V/K/H antibody (Cat#62056, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
