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KD-Validated STAT1 Mouse mAb

#62066

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Knockdown Validated
Western blotting analysis using STAT1 antibody (Cat#62066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Western blotting analysis using STAT1 antibody (Cat#62066). STAT1 expression in wild type (WT) and STAT1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    STAT1
  • Catalog:
    62066
  • Application:
    WB
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated STAT1 Mouse mAb
Aliases STAT1; Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; STAT91; ISGF-3; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; CANDF7; IMD31A; IMD31B; IMD31C
Background

Gene Name: STAT1

NCBI Gene Entry: 6772

UniProt Entry: P42224

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 87 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9460

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human STAT1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using STAT1 antibody (Cat#62066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
  • Western blotting analysis using STAT1 antibody (Cat#62066). STAT1 expression in wild type (WT) and STAT1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using STAT1 antibody (Cat#62066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Western blotting analysis using STAT1 antibody (Cat#62066). STAT1 expression in wild type (WT) and STAT1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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