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KD-Validated PBK Mouse mAb

#62179

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shRNA Knockdown Validated
Western blotting analysis using PBK antibody (Cat#62179). PBK expression in wild-type (WT) and PBK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PBK antibody (Cat#62179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PBK
  • Catalog:
    62179
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated PBK Mouse mAb
Aliases PBK; PDZ Binding Kinase; Nori-3; TOPK; CT84; SPK; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Lymphokine-Activated Killer T-Cell-Originated Protein Kinase; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; FLJ14385; Serine/Threonine Protein Kinase; Epididymis Luminal Protein 164; PDZ-Binding Kinase; EC 2.7.12.2; HEL164
Background

Gene Name: PBK

NCBI Gene Entry: 55872

UniProt Entry: Q96KB5

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 38 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1695

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human PBK
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PBK antibody (Cat#62179). PBK expression in wild-type (WT) and PBK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PBK antibody (Cat#62179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PBK antibody (Cat#62179). PBK expression in wild-type (WT) and PBK shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PBK antibody (Cat#62179, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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