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KD-Validated ESR1 Recombinant Rabbit mAb

#62187

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using ESR1 antibody (Cat#62187). ESR1 expression in wild type (WT) and ESR1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ESR1 antibody (Cat#62187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ESR1
  • Catalog:
    62187
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated ESR1 Recombinant Rabbit mAb
Aliases ESR1; Estrogen Receptor 1; ER-Alpha; NR3A1; ER; Nuclear Receptor Subfamily 3 Group A Member 1; Estradiol Receptor; Era; ESR; Oestrogen Receptor Alpha; E2 Receptor Alpha; Estrogen Receptor; Estrogen Receptor Alpha E1-N2-E2-1-2; Estrogen Receptor Alpha E1-E2-1-2; Estrogen Nuclear Receptor Alpha; Estrogen Receptor Alpha; ESTRR; ESRA
Background

Gene Name: ESR1

NCBI Gene Entry: 2099

UniProt Entry: P03372

Application Information

Molecular Weight: Predicted, 66 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1725

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human ESR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using ESR1 antibody (Cat#62187). ESR1 expression in wild type (WT) and ESR1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ESR1 antibody (Cat#62187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ESR1 antibody (Cat#62187). ESR1 expression in wild type (WT) and ESR1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ESR1 antibody (Cat#62187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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