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Home>Product>Primary Antibody

KD-Validated HDHD5 Recombinant Rabbit mAb

#62225

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using HDHD5 antibody (Cat#62225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDHD5 antibody (Cat#62225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HDHD5 antibody (Cat#62225). HDHD5 expression in wild type (WT) and HDHD5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HDHD5 antibody (Cat#62225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using HDHD5 antibody (Cat#62225, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with HDHD5 antibody (Cat#62225, 1:1,000). Nuclei were stained blue with DAPI; HDHD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using HDHD5 antibody (Cat#62225, 1:1,000), Top panel: wild-type (WT); Bottom panal: HDHD5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HDHD5 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of HDHD5 expression in HepG2 cells using HDHD5 antibody (Cat#62225, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HDHD5.
Validation of HDHD5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HDHD5 antibody (Cat#62225, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    HDHD5
  • Catalog：
    62225
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated HDHD5 Recombinant Rabbit mAb
Aliases HDHD5; Haloacid Dehalogenase Like Hydrolase Domain Containing 5; CECR5; Haloacid Dehalogenase-Like Hydrolase Domain-Containing 5; Cat Eye Syndrome Chromosome Region, Candidate 5; Cat Eye Syndrome Critical Region Protein 5
Background

Gene Name: HDHD5

NCBI Gene Entry: 27440

UniProt Entry: Q9BXW7

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6585

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human CECR5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using HDHD5 antibody (Cat#62225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDHD5 antibody (Cat#62225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using HDHD5 antibody (Cat#62225). HDHD5 expression in wild type (WT) and HDHD5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HDHD5 antibody (Cat#62225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using HDHD5 antibody (Cat#62225, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with HDHD5 antibody (Cat#62225, 1:1,000). Nuclei were stained blue with DAPI; HDHD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using HDHD5 antibody (Cat#62225, 1:1,000), Top panel: wild-type (WT); Bottom panal: HDHD5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HDHD5 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of HDHD5 expression in HepG2 cells using HDHD5 antibody (Cat#62225, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HDHD5.
  • Validation of HDHD5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HDHD5 antibody (Cat#62225, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDHD5
  • Catalog：
    62225
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HDHD5 antibody (Cat#62225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDHD5 antibody (Cat#62225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HDHD5 antibody (Cat#62225). HDHD5 expression in wild type (WT) and HDHD5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HDHD5 antibody (Cat#62225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using HDHD5 antibody (Cat#62225, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with HDHD5 antibody (Cat#62225, 1:1,000). Nuclei were stained blue with DAPI; HDHD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using HDHD5 antibody (Cat#62225, 1:1,000), Top panel: wild-type (WT); Bottom panal: HDHD5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HDHD5 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of HDHD5 expression in HepG2 cells using HDHD5 antibody (Cat#62225, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HDHD5.
Validation of HDHD5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HDHD5 antibody (Cat#62225, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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