Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated Pyruvate Dehydrogenase E2 Recombinant Rabbit mAb

#62417

Click to copy product information

Return to List

Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Pyruvate Dehydrogenase E2 antibody (Cat#62417). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Pyruvate Dehydrogenase E2 antibody (Cat#62417). Pyruvate Dehydrogenase E2 expression in wild type (WT) and Pyruvate Dehydrogenase E2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:1,000). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:1,000), Top panel: wild-type (WT); Bottom panal: Pyruvate Dehydrogenase E2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of Pyruvate Dehydrogenase E2 expression in HepG2 cells using Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Pyruvate Dehydrogenase E2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DLAT
  • Catalog：
    62417
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Pyruvate Dehydrogenase E2 Recombinant Rabbit mAb
Aliases DLAT; Dihydrolipoamide S-Acetyltransferase; PBC;DLTA; PDC-E2; PDCE2; M2 Antigen Complex 70 KDa Subunit; E2 Component Of Pyruvate Dehydrogenase Complex; EC 2.3.1.12; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex Mitochondrial; Pyruvate Dehydrogenase Complex Component E2; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; EC 2.3.1
Background

Gene Name: DLAT

NCBI Gene Entry: 1737

UniProt Entry: P10515

Application Information

Molecular Weight: Predicted, 69 kDa; observed, 69 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1010

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Pyruvate Dehydrogenase E2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Pyruvate Dehydrogenase E2 antibody (Cat#62417). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Pyruvate Dehydrogenase E2 antibody (Cat#62417). Pyruvate Dehydrogenase E2 expression in wild type (WT) and Pyruvate Dehydrogenase E2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:1,000). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:1,000), Top panel: wild-type (WT); Bottom panal: Pyruvate Dehydrogenase E2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
  • Flow cytometric analysis of Pyruvate Dehydrogenase E2 expression in HepG2 cells using Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Pyruvate Dehydrogenase E2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DLAT
  • Catalog：
    62417
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Pyruvate Dehydrogenase E2 antibody (Cat#62417). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Pyruvate Dehydrogenase E2 antibody (Cat#62417). Pyruvate Dehydrogenase E2 expression in wild type (WT) and Pyruvate Dehydrogenase E2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:1,000). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:1,000), Top panel: wild-type (WT); Bottom panal: Pyruvate Dehydrogenase E2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of Pyruvate Dehydrogenase E2 expression in HepG2 cells using Pyruvate Dehydrogenase E2 antibody (Cat#62417, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Pyruvate Dehydrogenase E2.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.