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Home>Product>Primary Antibody

KD-Validated NIMA Related Kinase 6 Recombinant Rabbit mAb

#62421

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using NEK6 antibody (Cat#62421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NEK6 antibody (Cat#62421). NEK6 expression in wild type (WT) and NEK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with NEK6 antibody (Cat#62421, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using NEK6 antibody (Cat#62421, 1:1,000), Top panel: wild-type (WT); Bottom panal: NEK6 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of NEK6 expression in HT-1080 cells using NEK6 antibody(Cat#62421, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NEK6.
Validation of NEK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NEK6 antibody (Cat#62421, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEK6
  • Catalog：
    62421
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated NIMA Related Kinase 6 Recombinant Rabbit mAb
Aliases NIMA Related Kinase 6; SID6-1512; NIMA (Never In Mitosis Gene A)-Related Kinase 6; Putative Serine-Threonine Protein Kinase; Serine/Threonine-Protein Kinase Nek6; Never In Mitosis A-Related Kinase 6; NimA-Related Protein Kinase 6; Protein Kinase SID6-1512; EC 2.7.11.34; EC 2.7.11.1
Background

Gene Name: NEK6

NCBI Gene Entry: 10783

UniProt Entry: Q9HC98

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB755

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NEK6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NEK6 antibody (Cat#62421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NEK6 antibody (Cat#62421). NEK6 expression in wild type (WT) and NEK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with NEK6 antibody (Cat#62421, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using NEK6 antibody (Cat#62421, 1:1,000), Top panel: wild-type (WT); Bottom panal: NEK6 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of NEK6 expression in HT-1080 cells using NEK6 antibody(Cat#62421, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NEK6.
  • Validation of NEK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NEK6 antibody (Cat#62421, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEK6
  • Catalog：
    62421
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NEK6 antibody (Cat#62421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NEK6 antibody (Cat#62421). NEK6 expression in wild type (WT) and NEK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with NEK6 antibody (Cat#62421, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using NEK6 antibody (Cat#62421, 1:1,000), Top panel: wild-type (WT); Bottom panal: NEK6 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of NEK6 expression in HT-1080 cells using NEK6 antibody(Cat#62421, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NEK6.
Validation of NEK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NEK6 antibody (Cat#62421, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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