KD-Validated TRK Fused Gene Recombinant Rabbit mAb - Product

KD-Validated TRK Fused Gene Recombinant Rabbit mAb

#62630

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using TFG antibody (Cat#62630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFG antibody (Cat#62630, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using TFG antibody (Cat#62630). TFG expression in wild type (WT) and TFG shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TFG antibody (Cat#62630, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunocytochemical staining of HepG2 cells with TFG antibody (Cat#62630, 1:1,000). Nuclei were stained blue with DAPI; TFG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.

Immunocytochemical staining of HeLa cells using TFG antibody (Cat#62630, 1:1,000), Top panel: wild-type (WT); Bottom panal: TFG shRNA knockdown (KD). Nuclei were stained blue with DAPI; TFG was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.

Flow cytometric analysis of TFG expression in HepG2 cells using TFG antibody (Cat#62630, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TFG.

Validation of TFG knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TFG antibody (Cat#62630, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TFG
Catalog：

62630
Application：

WB, FCM, ICC
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated TRK Fused Gene Recombinant Rabbit mAb
Aliases	TFG; Trafficking From ER To Golgi Regulator; SPG57; TF6; TRK-Fused Gene Protein; Protein TFG; FLJ36137; TRK-Fused Gene; TRKT3 Oncogene; TRK-Fused;HMSNP; TRKT3
Background	Gene Name: TFG NCBI Gene Entry: 10342 UniProt Entry: Q92734
Application Information	Molecular Weight: Predicted, 43 kDa; observed, 55 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2115 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen	A synthesized peptide derived from human TRK fused gene
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using TFG antibody (Cat#62630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFG antibody (Cat#62630, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TFG antibody (Cat#62630). TFG expression in wild type (WT) and TFG shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TFG antibody (Cat#62630, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TFG antibody (Cat#62630, 1:1,000). Nuclei were stained blue with DAPI; TFG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using TFG antibody (Cat#62630, 1:1,000), Top panel: wild-type (WT); Bottom panal: TFG shRNA knockdown (KD). Nuclei were stained blue with DAPI; TFG was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of TFG expression in HepG2 cells using TFG antibody (Cat#62630, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TFG.
Validation of TFG knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TFG antibody (Cat#62630, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

TFG
Catalog：

62630
Application：

WB, FCM, ICC
Species Reactivity：

H, M, R

Datasheet

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