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Home>Product>Primary Antibody

KD-Validated Integrin Subunit Alpha 5 Rabbit pAb

#62822

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shRNA Knockdown Validated
Western blotting analysis using Integrin subunit alpha 5 antibody (Cat#62822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha 5 antibody (Cat#62822, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin subunit alpha 5 antibody (Cat#62822). Integrin subunit alpha 5 expression in wild type (WT) and Integrin subunit alpha 5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin subunit alpha 5 antibody (Cat#62822, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITGA5
  • Catalog：
    62822
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Integrin Subunit Alpha 5 Rabbit pAb
Aliases ITGA5; Integrin Subunit Alpha 5; CD49e; FNRA; Integrin, Alpha 5 (Fibronectin Receptor, Alpha Polypeptide); Fibronectin Receptor, Alpha Polypeptide; Fibronectin Receptor Subunit Alpha; CD49 Antigen-Like Family Member E; Integrin Alpha-5; Integrin Alpha-F; VLA-5; Very Late Activation Protein 5, Alpha Subunit; Fibronectin Receptor, Alpha Subunit; ITGA5/SLC9B1 Fusion; CD49e Antigen; VLA5A
Background

Gene Name: ITGA5

NCBI Gene Entry: 3678

UniProt Entry: P08648

Application Information

Molecular Weight: Predicted, 115 kDa; observed, 18 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human CD49e LC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using Integrin subunit alpha 5 antibody (Cat#62822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha 5 antibody (Cat#62822, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Integrin subunit alpha 5 antibody (Cat#62822). Integrin subunit alpha 5 expression in wild type (WT) and Integrin subunit alpha 5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin subunit alpha 5 antibody (Cat#62822, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITGA5
  • Catalog：
    62822
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Integrin subunit alpha 5 antibody (Cat#62822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha 5 antibody (Cat#62822, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin subunit alpha 5 antibody (Cat#62822). Integrin subunit alpha 5 expression in wild type (WT) and Integrin subunit alpha 5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin subunit alpha 5 antibody (Cat#62822, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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