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Home>Product>Primary Antibody

KD-Validated NR3C1 Mouse mAb

#62834

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shRNA Knockdown Validated
Western blotting analysis using NR3C1 antibody (Cat#62834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR3C1 antibody (Cat#62834, 1:5,000) and HRP-conjugated goat anti mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NR3C1 antibody (Cat#62834). Glucocorticoid Receptor expression in wild type (WT) and NR3C1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NR3C1 antibody (Cat#62834, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NR3C1
  • Catalog：
    62834
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NR3C1 Mouse mAb
Aliases NR3C1; Nuclear Receptor Subfamily 3 Group C Member 1; GR; Glucocorticoid Receptor; GRL; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); Nuclear Receptor Subfamily 3 Group C Member 1 Variant HGR-B(54); Nuclear Receptor Subfamily 3 Group C Member 1 Variant HGR-B(77); Nuclear Receptor Subfamily 3 Group C Member 1 Variant HGR-B(93); Nuclear Receptor Subfamily 3, Group C, Member 1; GCRST; GCCR; GCR
Background

Gene Name: NR3C1

NCBI Gene Entry: 2908

UniProt Entry: P04150

Application Information

Molecular Weight: Predicted, 86 kDa; observed, 94 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2760

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human Glucocorticoid Receptor
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using NR3C1 antibody (Cat#62834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR3C1 antibody (Cat#62834, 1:5,000) and HRP-conjugated goat anti mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NR3C1 antibody (Cat#62834). Glucocorticoid Receptor expression in wild type (WT) and NR3C1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NR3C1 antibody (Cat#62834, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NR3C1
  • Catalog：
    62834
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using NR3C1 antibody (Cat#62834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR3C1 antibody (Cat#62834, 1:5,000) and HRP-conjugated goat anti mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NR3C1 antibody (Cat#62834). Glucocorticoid Receptor expression in wild type (WT) and NR3C1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NR3C1 antibody (Cat#62834, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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