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Home>Product>Primary Antibody

KD-Validated Superoxide Dismutase 2 Mouse mAb

#62883

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shRNA Knockdown Validated
Western blotting analysis using Superoxide dismutase 2 antibody (Cat#62883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Superoxide dismutase 2 antibody (Cat#62883, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using superoxide dismutase 2 antibody (Cat#62883). Superoxide dismutase 2 expression in wild-type (WT) and superoxide dismutase 2 (SOD2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with superoxide dismutase 2 antibody (Cat#62883, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SOD2
  • Catalog：
    62883
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Superoxide Dismutase 2 Mouse mAb
Aliases SOD2; Superoxide Dismutase 2; GClnc1; MnSOD; IPOB; GC1; Superoxide Dismutase [Mn], Mitochondrial; Superoxide Dismutase 2, Mitochondrial; EC 1.15.1.1; Epididymis Secretory Sperm Binding Protein; Manganese-Containing Superoxide Dismutase; Gastric Cancer–Associated LncRNA 1; Gastric Cancer-Associated LncRNA 1; Manganese Superoxide Dismutase; Mangano-Superoxide Dismutase; Mn Superoxide Dismutase; Indophenoloxidase B; Mn-SOD; IPO-B; MVCD6
Background

Gene Name: SOD2

NCBI Gene Entry: 6648

UniProt Entry: P04179

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 22 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2990

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human SOD2
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using Superoxide dismutase 2 antibody (Cat#62883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Superoxide dismutase 2 antibody (Cat#62883, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using superoxide dismutase 2 antibody (Cat#62883). Superoxide dismutase 2 expression in wild-type (WT) and superoxide dismutase 2 (SOD2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with superoxide dismutase 2 antibody (Cat#62883, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SOD2
  • Catalog：
    62883
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Superoxide dismutase 2 antibody (Cat#62883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Superoxide dismutase 2 antibody (Cat#62883, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using superoxide dismutase 2 antibody (Cat#62883). Superoxide dismutase 2 expression in wild-type (WT) and superoxide dismutase 2 (SOD2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with superoxide dismutase 2 antibody (Cat#62883, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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