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Home>Product>Primary Antibody

PLEKHO2 Mouse mAb

#6319

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Western blotting analysis using PLEKHO2 antibody (Cat#6319). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PLEKHO2 antibody (Cat#6319, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Raji cells with PLEKHO2 antibody (Cat#6319, 1:1,000). Nuclei were stained blue with DAPI; PLEKHO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PLEKHO2 expression in Raji cells using PLEKHO2 antibody (Cat#6319, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PLEKHO2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PLEKHO2
  • Catalog：
    6319
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name PLEKHO2 Mouse mAb
Aliases PLEKHO2; Pleckstrin Homology Domain Containing O2; PLEKHQ1; PP1628; Pp9099; Pleckstrin Homology Domain-Containing Family O Member 2; Pleckstrin Homology Domain-Containing Family Q Member 1; PH Domain-Containing Family O Member 2; PH Domain-Containing Family Q Member 1; DKFZp761K2312; Pleckstrin Homology Domain Containing, Family Q Member 1; Pleckstrin Homology Domain Containing, Family O Member 2; PH Domain-Containing Protein
Background

Gene Name: PLEKHO2

NCBI Gene Entry: 80301

UniProt Entry: Q8TD55

Application Information

Molecular Weight: Predicted, 53 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8555

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PLEKHO2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PLEKHO2 antibody (Cat#6319). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PLEKHO2 antibody (Cat#6319, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Raji cells with PLEKHO2 antibody (Cat#6319, 1:1,000). Nuclei were stained blue with DAPI; PLEKHO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PLEKHO2 expression in Raji cells using PLEKHO2 antibody (Cat#6319, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PLEKHO2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PLEKHO2
  • Catalog：
    6319
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PLEKHO2 antibody (Cat#6319). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PLEKHO2 antibody (Cat#6319, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Raji cells with PLEKHO2 antibody (Cat#6319, 1:1,000). Nuclei were stained blue with DAPI; PLEKHO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PLEKHO2 expression in Raji cells using PLEKHO2 antibody (Cat#6319, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PLEKHO2.
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