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Home>Product>Primary Antibody

KD-Validated HDAC3 Recombinant Rabbit mAb

#63243

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using HDAC3 antibody (Cat#63243). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HDAC3 antibody (Cat#63243). HDAC3 expression in wild type (WT) and HDAC3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse brain using HDAC3 antibody (Cat#63243, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with HDAC3 antibody (Cat#63243, 1:1,000). Nuclei were stained blue with DAPI; HDAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HDAC3 expression in HepG2 cells using HDAC3 antibody (Cat#63243,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HDAC3.
Validation of HDAC3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HDAC3 antibody (Cat#63243, 1:2,000) and analyzed using CytoFLEX. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDAC3
  • Catalog：
    63243
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated HDAC3 Recombinant Rabbit mAb
Aliases Histone Deacetylase 3; RPD3-2; HD3 KDAC3; RPD3; Protein Deacetylase HDAC3; Protein Deacylase HDAC3; EC 3.5.1.98; SMAP45; EC 3.5.1.-
Background

Gene Name: HDAC3

NCBI Gene Entry: 8841

UniProt Entry: O15379

Application Information

Molecular Weight: Predicted, 49 kDa; observed, 49 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB860

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human HDAC3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using HDAC3 antibody (Cat#63243). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using HDAC3 antibody (Cat#63243). HDAC3 expression in wild type (WT) and HDAC3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using HDAC3 antibody (Cat#63243, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with HDAC3 antibody (Cat#63243, 1:1,000). Nuclei were stained blue with DAPI; HDAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HDAC3 expression in HepG2 cells using HDAC3 antibody (Cat#63243,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HDAC3.
  • Validation of HDAC3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HDAC3 antibody (Cat#63243, 1:2,000) and analyzed using CytoFLEX. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDAC3
  • Catalog：
    63243
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using HDAC3 antibody (Cat#63243). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HDAC3 antibody (Cat#63243). HDAC3 expression in wild type (WT) and HDAC3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HDAC3 antibody (Cat#63243, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse brain using HDAC3 antibody (Cat#63243, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with HDAC3 antibody (Cat#63243, 1:1,000). Nuclei were stained blue with DAPI; HDAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HDAC3 expression in HepG2 cells using HDAC3 antibody (Cat#63243,1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HDAC3.
Validation of HDAC3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HDAC3 antibody (Cat#63243, 1:2,000) and analyzed using CytoFLEX. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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