KD-Validated Cofilin 1 Recombinant Rabbit mAb - Product

KD-Validated Cofilin 1 Recombinant Rabbit mAb

#63264

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using cofilin 1 antibody (Cat#63264). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cofilin 1 antibody (Cat#63264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using cofilin 1 antibody (Cat#63264). Cofilin 1 expression in wild type (WT) and cofilin 1 (CFL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with cofilin 1 antibody (Cat#63264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of cofilin 1 expression in H9c2 cells using cofilin 1 antibody (Cat#63264, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, cofilin 1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CFL1
Catalog：

63264
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated Cofilin 1 Recombinant Rabbit mAb
Aliases	CFL1; Cofilin 1; CFL; Cofilin 1 (Non-Muscle); 18 KDa Phosphoprotein; Cofilin-1; P18; Epididymis Secretory Protein Li 15; Cofilin, Non-Muscle Isoform; HEL-S-15; Cofilin
Background	Gene Name: CFL1 NCBI Gene Entry: 1072 UniProt Entry: P23528
Application Information	Molecular Weight: Predicted, 19 kDa; observed, 19 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4775 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Cofilin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using cofilin 1 antibody (Cat#63264). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cofilin 1 antibody (Cat#63264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cofilin 1 antibody (Cat#63264). Cofilin 1 expression in wild type (WT) and cofilin 1 (CFL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with cofilin 1 antibody (Cat#63264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cofilin 1 expression in H9c2 cells using cofilin 1 antibody (Cat#63264, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, cofilin 1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CFL1
Catalog：

63264
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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