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Home>Product>Primary Antibody

KD-Validated Cystathionine Gamma-Lyase Recombinant Rabbit mAb

#63268

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Cystathionine gamma-lyase expression in wild type (WT) and cystathionine gamma-lyase (CTH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cystathionine gamma-lyase expression in HepG2 cells using cystathionine gamma-lyase antibody (Cat#63268, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cystathionine Gamma-Lyase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CTH
  • Catalog：
    63268
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Cystathionine Gamma-Lyase Recombinant Rabbit mAb
Aliases CTH; Cystathionine Gamma-Lyase; CSE; Cystathionase (Cystathionine Gamma-Lyase); Cysteine-Protein Sulfhydrase; Homocysteine Desulfhydrase; Cysteine Desulfhydrase; Gamma-Cystathionase; EC 4.4.1.1; CGL; Homoserine Dehydratase; Homoserine Deaminase; EC 4.4.1.2; EC 4.4.1
Background

Gene Name: CTH

NCBI Gene Entry: 1491

UniProt Entry: P32929

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4795

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Cystathionase/CTH
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Cystathionine gamma-lyase expression in wild type (WT) and cystathionine gamma-lyase (CTH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of cystathionine gamma-lyase expression in HepG2 cells using cystathionine gamma-lyase antibody (Cat#63268, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cystathionine Gamma-Lyase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CTH
  • Catalog：
    63268
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Cystathionine gamma-lyase expression in wild type (WT) and cystathionine gamma-lyase (CTH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cystathionine gamma-lyase expression in HepG2 cells using cystathionine gamma-lyase antibody (Cat#63268, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cystathionine Gamma-Lyase.
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