Immunocytochemical staining of HAP-1 cells with E2F transcription factor 4 antibody (Cat#63375, 1:1,000). Nuclei were stained blue with DAPI;E2F transcription factor 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of E2F transcription factor 4 expression in HAP-1 cells using E2F transcription factor 4 antibody (Cat#63375, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, E2F transcription factor 4.
Western blotting analysis using E2F transcription factor 4 antibody (Cat#63375). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with E2F transcription factor 4 antibody (Cat#63375, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using E2F transcription factor 4 antibody (Cat#63375). E2F transcription factor 4 expression in wild type (WT) and E2F transcription factor 4 (E2F4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with E2F transcription factor 4 antibody (Cat#63375, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
