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Home>Product>Primary Antibody

KD-Validated YY1 Mouse mAb

#63709

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shRNA Knockdown Validated
Western blotting analysis using YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of YY1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with YY1 antibody (Cat#63709, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YY1
  • Catalog：
    63709
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated YY1 Mouse mAb
Aliases YY1; YY1 Transcription Factor; INO80S; NF-E1; INO80 Complex Subunit S; YIN-YANG-1; DELTA; UCRBP; Transcriptional Repressor Protein YY1; Delta Transcription Factor; Yin And Yang 1 Protein; YY-1; Yin And Yang 1; GADEVS
Background

Gene Name: YY1

NCBI Gene Entry: 7528

UniProt Entry: P25490

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7020

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human YY1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of YY1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with YY1 antibody (Cat#63709, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YY1
  • Catalog：
    63709
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of YY1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with YY1 antibody (Cat#63709, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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