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Home>Product>Primary Antibody

KD-Validated DNM1L Mouse mAb

#63857

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shRNA Knockdown Validated
Western blotting analysis using dynamin 1 like antibody (Cat#63857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dynamin 1 like antibody (Cat#63857, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using dynamin 1 like antibody (Cat#63857). Dynamin 1 like expression in wild type (WT) and dynamin 1 like (DNM1L) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dynamin 1 like antibody (Cat#63857, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Dynamin 1 like protein antibody (Cat#63857, 1:500). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Dynamin 1 like antibody (Cat#63857, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dynamin 1 like shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Dynamin 1 like expression in HepG2 cells using Dynamin 1 like antibody (Cat#63857, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Dynamin 1 like.
Validation of Dynamin 1 like knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dynamin 1 like antibody (Cat#63857, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    DNM1L
  • Catalog：
    63857
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated DNM1L Mouse mAb
Aliases DNM1L; Dynamin 1 Like; DRP1; DVLP; HDYNIV; DYMPLE; Dynamin Family Member Proline-Rich Carboxyl-Terminal Domain Less; Dynamin-Related Protein 1; Dnm1p/Vps1p-Like Protein; Dynamin-Like Protein IV; Dynamin-1-Like Protein; Dynamin-Like Protein 4; EC 3.6.5.5; VPS1; DLP1; Dynamin-Like Protein; Dymple; HdynIV; EMPF1; EMPF; OPA5
Background

Gene Name: DNM1L

NCBI Gene Entry: 10059

UniProt Entry: O00429

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 74 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8215

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human DNM1L
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using dynamin 1 like antibody (Cat#63857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dynamin 1 like antibody (Cat#63857, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using dynamin 1 like antibody (Cat#63857). Dynamin 1 like expression in wild type (WT) and dynamin 1 like (DNM1L) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dynamin 1 like antibody (Cat#63857, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Dynamin 1 like protein antibody (Cat#63857, 1:500). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HeLa cells using Dynamin 1 like antibody (Cat#63857, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dynamin 1 like shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Dynamin 1 like expression in HepG2 cells using Dynamin 1 like antibody (Cat#63857, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Dynamin 1 like.
  • Validation of Dynamin 1 like knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dynamin 1 like antibody (Cat#63857, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DNM1L
  • Catalog：
    63857
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using dynamin 1 like antibody (Cat#63857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dynamin 1 like antibody (Cat#63857, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using dynamin 1 like antibody (Cat#63857). Dynamin 1 like expression in wild type (WT) and dynamin 1 like (DNM1L) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dynamin 1 like antibody (Cat#63857, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Dynamin 1 like protein antibody (Cat#63857, 1:500). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Dynamin 1 like antibody (Cat#63857, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dynamin 1 like shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Dynamin 1 like expression in HepG2 cells using Dynamin 1 like antibody (Cat#63857, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Dynamin 1 like.
Validation of Dynamin 1 like knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dynamin 1 like antibody (Cat#63857, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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