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Home>Product>Primary Antibody

KD-Validated FAF1 Mouse mAb

#63863

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shRNA Knockdown Validated
Western blotting analysis using fas associated factor 1 antibody (Cat#63863). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fas associated factor 1 antibody (Cat#63863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using fas associated factor 1 antibody (Cat#63863). Fas associated factor 1 expression in wild type (WT) and fas associated factor 1 (FAF1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with fas associated factor 1 antibody (Cat#63863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Fas associated factor 1 antibody (Cat#63863, 1:500). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Fas associated factor 1 antibody (Cat#63863, 1:1,000), Top panel: wild-type (WT); Bottom panal: Fas associated factor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Fas associated factor 1 expression in HepG2 cells using Fas associated factor 1 antibody (Cat#63863, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fas associated factor 1.
Validation of Fas associated factor 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Fas associated factor 1 antibody (Cat#63863, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FAF1
  • Catalog：
    63863
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated FAF1 Mouse mAb
Aliases FAF1; Fas Associated Factor 1; UBXD12; UBXN3A; HFAF1; CGI-03; HFAF1s; Fas (TNFRSF6) Associated Factor 1; UBX Domain-Containing Protein 12; UBX Domain-Containing Protein 3A; TNFRSF6-Associated Factor 1; FAS-Associated Factor 1; UBX Domain Protein 3A
Background

Gene Name: FAF1

NCBI Gene Entry: 11124

UniProt Entry: Q9UNN5

Application Information

Molecular Weight: Predicted, 74 kDa; observed, 74 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8245

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human FAF1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using fas associated factor 1 antibody (Cat#63863). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fas associated factor 1 antibody (Cat#63863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using fas associated factor 1 antibody (Cat#63863). Fas associated factor 1 expression in wild type (WT) and fas associated factor 1 (FAF1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with fas associated factor 1 antibody (Cat#63863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Fas associated factor 1 antibody (Cat#63863, 1:500). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HeLa cells using Fas associated factor 1 antibody (Cat#63863, 1:1,000), Top panel: wild-type (WT); Bottom panal: Fas associated factor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Fas associated factor 1 expression in HepG2 cells using Fas associated factor 1 antibody (Cat#63863, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fas associated factor 1.
  • Validation of Fas associated factor 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Fas associated factor 1 antibody (Cat#63863, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FAF1
  • Catalog：
    63863
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using fas associated factor 1 antibody (Cat#63863). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fas associated factor 1 antibody (Cat#63863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using fas associated factor 1 antibody (Cat#63863). Fas associated factor 1 expression in wild type (WT) and fas associated factor 1 (FAF1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with fas associated factor 1 antibody (Cat#63863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Fas associated factor 1 antibody (Cat#63863, 1:500). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Fas associated factor 1 antibody (Cat#63863, 1:1,000), Top panel: wild-type (WT); Bottom panal: Fas associated factor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Fas associated factor 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Fas associated factor 1 expression in HepG2 cells using Fas associated factor 1 antibody (Cat#63863, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Fas associated factor 1.
Validation of Fas associated factor 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Fas associated factor 1 antibody (Cat#63863, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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