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Home>Product>Primary Antibody

KD-Validated FANCC Mouse mAb

#63882

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shRNA Knockdown Validated
Western blotting analysis using FANCC antibody (Cat#63882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FANCC antibody (Cat#63882, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FANCC antibody (Cat#63882). FANCC expression in wild type (WT) and FANCC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FANCC antibody (Cat#63882, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of FANCC knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with FANCC antibody (Cat#63882, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FANCC
  • Catalog：
    63882
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated FANCC Mouse mAb
Aliases FANCC; FA Complementation Group C; FAC; FACC; FA3; Fanconi Anemia Complementation Group C; Fanconi Anemia Group C Protein; Protein FACC
Background

Gene Name: FANCC

NCBI Gene Entry: 2176

UniProt Entry: Q00597

Application Information

Molecular Weight: Predicted, 63 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8340

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human FANCC
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using FANCC antibody (Cat#63882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FANCC antibody (Cat#63882, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using FANCC antibody (Cat#63882). FANCC expression in wild type (WT) and FANCC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FANCC antibody (Cat#63882, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of FANCC knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with FANCC antibody (Cat#63882, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FANCC
  • Catalog：
    63882
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using FANCC antibody (Cat#63882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FANCC antibody (Cat#63882, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FANCC antibody (Cat#63882). FANCC expression in wild type (WT) and FANCC shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FANCC antibody (Cat#63882, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of FANCC knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with FANCC antibody (Cat#63882, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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