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Home>Product>Primary Antibody

KD-Validated PI3 Kinase p110 Beta Recombinant Rabbit mAb

#64311

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PI3 Kinase p110 beta antibody (Cat#64311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PI3 Kinase p110 beta antibody (Cat#64311). PI3 Kinase p110 beta expression in wild type (WT) and PI3 Kinase p110 beta shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000), Top panel: wild-type (WT); Bottom panal: PI3 Kinase p110 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of PI3 Kinase p110 beta expression in HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 Kinase p110 beta.
Validation of PI3 Kinase p110 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIK3CB
  • Catalog：
    64311
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated PI3 Kinase p110 Beta Recombinant Rabbit mAb
Aliases Phosphatidylinositol-4,5-Bisphosphate 3-Kinase; Catalytic SubunitBeta; PI3K; PIK3C1; PI3KBETA; Phosphoinositide-3-Kinase; Catalytic; Beta Polypeptide; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit Beta Isoform; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Beta; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Beta; PtdIns-3-Kinase Subunit Beta; PI3-Kinase P110 Subunit Beta; PtdIns-3-Kinase Subunit P110-Beta; PtdIns-3-Kinase P110; PI3K-beta; PI3Kbeta; PI3-Kinase Subunit Beta; p110beta; EC 2.7.1.153; PI3KCB; P110BETA; EC 2.7.1
Background

Gene Name: PIK3CB

NCBI Gene Entry: 5291

UniProt Entry: P42338

Application Information

Molecular Weight: Predicted, 123 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1035

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PI3 Kinase p110 beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PI3 Kinase p110 beta antibody (Cat#64311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using PI3 Kinase p110 beta antibody (Cat#64311). PI3 Kinase p110 beta expression in wild type (WT) and PI3 Kinase p110 beta shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000), Top panel: wild-type (WT); Bottom panal: PI3 Kinase p110 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of PI3 Kinase p110 beta expression in HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 Kinase p110 beta.
  • Validation of PI3 Kinase p110 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIK3CB
  • Catalog：
    64311
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PI3 Kinase p110 beta antibody (Cat#64311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PI3 Kinase p110 beta antibody (Cat#64311). PI3 Kinase p110 beta expression in wild type (WT) and PI3 Kinase p110 beta shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PI3 Kinase p110 beta antibody (Cat#64311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:1,000), Top panel: wild-type (WT); Bottom panal: PI3 Kinase p110 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of PI3 Kinase p110 beta expression in HT-1080 cells using PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PI3 Kinase p110 beta.
Validation of PI3 Kinase p110 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with PI3 Kinase p110 beta antibody (Cat#64311, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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