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Home>Product>Primary Antibody

NDUFB8 Mouse mAb

#6437

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Western blotting analysis using NDUFB8 antibody (Cat#6437). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB8 antibody (Cat#6437, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with NDUFB8 antibody (Cat#6437, 1:1,000). Nuclei were stained blue with DAPI; NDUFB8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFB8 expression in HeLa cells using NDUFB8 antibody (Cat#6437, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFB8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFB8
  • Catalog：
    6437
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name NDUFB8 Mouse mAb
Aliases NDUFB8; NADH:Ubiquinone Oxidoreductase Subunit B8; CI-ASHI; ASHI; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 8, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 8, 19kDa; Complex I ASHI Subunit; Complex I-ASHI; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 8 (19kD, ASHI); NADH:Ubiquinone Oxidoreductase ASHI Subunit; NADH-Ubiquinone Oxidoreductase ASHI Subunit; MC1DN32
Background

Gene Name: NDUFB8

NCBI Gene Entry: 4714

UniProt Entry: O95169

Application Information

Molecular Weight: Predicted, 22 kDa; observed, 19 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9090

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human NDUFB8
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFB8 antibody (Cat#6437). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB8 antibody (Cat#6437, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with NDUFB8 antibody (Cat#6437, 1:1,000). Nuclei were stained blue with DAPI; NDUFB8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NDUFB8 expression in HeLa cells using NDUFB8 antibody (Cat#6437, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFB8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFB8
  • Catalog：
    6437
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NDUFB8 antibody (Cat#6437). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB8 antibody (Cat#6437, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with NDUFB8 antibody (Cat#6437, 1:1,000). Nuclei were stained blue with DAPI; NDUFB8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NDUFB8 expression in HeLa cells using NDUFB8 antibody (Cat#6437, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFB8.
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