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Home>Product>Primary Antibody

KD-Validated DDOST Mouse mAb

#64820

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shRNA Knockdown Validated
Western blotting analysis using DDOST antibody (Cat#64820). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDOST antibody (Cat#64820, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDOST antibody (Cat#64820). DDOST expression in wild-type (WT) and DDOST shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDOST antibody (Cat#64820, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of DDOST knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDOST antibody (Cat#64820, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDOST
  • Catalog：
    64820
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated DDOST Mouse mAb
Aliases DDOST; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Non-Catalytic Subunit; OST48; KIAA0115; WBP1; OST; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 48 KDa Subunit; Advanced Glycation End-Product Receptor 1; Oligosaccharyl Transferase 48 KDa Subunit; Oligosaccharyltransferase Subunit 48; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit (Non-Catalytic); Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase; Dolichyl-Diphosphooligosaccharide-Protein Glycotransferase; Advanced Glycation Endproduct Receptor 1; Oligosaccharyltransferase 48 KDa Subunit; DDOST 48 KDa Subunit; EC 2.4.1.119; OKSWcl45; AGER1; CDG1R; GATD6
Background

Gene Name: DDOST

NCBI Gene Entry: 1650

UniProt Entry: P39656

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 49 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13105

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human DDOST
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using DDOST antibody (Cat#64820). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDOST antibody (Cat#64820, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using DDOST antibody (Cat#64820). DDOST expression in wild-type (WT) and DDOST shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDOST antibody (Cat#64820, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of DDOST knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDOST antibody (Cat#64820, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDOST
  • Catalog：
    64820
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using DDOST antibody (Cat#64820). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDOST antibody (Cat#64820, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDOST antibody (Cat#64820). DDOST expression in wild-type (WT) and DDOST shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDOST antibody (Cat#64820, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of DDOST knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DDOST antibody (Cat#64820, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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