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Home>Product>Primary Antibody

HSPB1 Mouse mAb

#6483

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Western blotting analysis using HSPB1 antibody (Cat#6483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPB1 antibody (Cat#6483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HSPB1 antibody (Cat#6483, 1:1,000). Nuclei were stained blue with DAPI; HSPB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HSPB1 expression in HepG2 cells using HSPB1 antibody (Cat#6483, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSPB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPB1
  • Catalog：
    6483
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name HSPB1 Mouse mAb
Aliases HSPB1; Heat Shock Protein Family B (Small) Member 1; HSP27; HSP28; Hs.76067; Hsp25; CMT2F; Heat Shock Protein Family B Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein 1; Heat Shock 27kD Protein 1; Heat Shock Protein Beta-1; 28 KDa Heat Shock Protein; Heat Shock 27 KDa Protein; SRP27; Epididymis Secretory Protein Li 102; HEL-S-102; HSP 27; HMN2B; HMND3; HspB1
Background

Gene Name: HSPB1

NCBI Gene Entry: 3315

UniProt Entry: P04792

Application Information

Molecular Weight: Predicted, 23 kDa; observed, 25 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9525

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HSPB1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HSPB1 antibody (Cat#6483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPB1 antibody (Cat#6483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with HSPB1 antibody (Cat#6483, 1:1,000). Nuclei were stained blue with DAPI; HSPB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HSPB1 expression in HepG2 cells using HSPB1 antibody (Cat#6483, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSPB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPB1
  • Catalog：
    6483
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HSPB1 antibody (Cat#6483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPB1 antibody (Cat#6483, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HSPB1 antibody (Cat#6483, 1:1,000). Nuclei were stained blue with DAPI; HSPB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HSPB1 expression in HepG2 cells using HSPB1 antibody (Cat#6483, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HSPB1.
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