Western blotting analysis using PSMA6 antibody (Cat#64853). Total cell lysates (15 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA6 antibody (Cat#64853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PSMA6 antibody (Cat#64853). PSMA6 expression in wild-type (WT) and PSMA6 shRNA knockdown (KD) HeLa cells with 15 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PSMA6 antibody (Cat#64853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with PSMA6 antibody (Cat#64853, 1:1,000). Nuclei were stained blue with DAPI; PSMA6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of PSMA6 expression in HepG2 cells using PSMA6 antibody (Cat#64853, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSMA6.
