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Home>Product>Primary Antibody

HDAC6 Mouse mAb

#6486

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Western blotting analysis using HDAC6 antibody (Cat#6486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC6 antibody (Cat#6486, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with HDAC6 antibody (Cat#6486, 1:1,000). Nuclei were stained blue with DAPI; HDAC6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HDAC6 expression in HAP-1 cells using HDAC6 antibody (Cat#6486, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HDAC6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDAC6
  • Catalog：
    6486
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name HDAC6 Mouse mAb
Aliases HDAC6; Histone Deacetylase 6; KIAA0901; PPP1R90; KDAC6; JM21; HD6; Protein Phosphatase 1, Regulatory Subunit 90; E3 Ubiquitin-Protein Ligase HDAC6; Tubulin-Lysine Deacetylase HDAC6; Alpha-Tubulin Deacetylase HDAC6; Protein Deacetylase HDAC6; FLJ16239; EC 3.5.1.98; EC 3.5.1.-; EC 2.3.2.-; CPBHM
Background

Gene Name: HDAC6

NCBI Gene Entry: 10013

UniProt Entry: Q9UBN7

Application Information

Molecular Weight: Predicted, 131 kDa; observed, 160 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9540

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HDAC6
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HDAC6 antibody (Cat#6486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC6 antibody (Cat#6486, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with HDAC6 antibody (Cat#6486, 1:1,000). Nuclei were stained blue with DAPI; HDAC6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HDAC6 expression in HAP-1 cells using HDAC6 antibody (Cat#6486, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HDAC6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDAC6
  • Catalog：
    6486
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HDAC6 antibody (Cat#6486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC6 antibody (Cat#6486, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with HDAC6 antibody (Cat#6486, 1:1,000). Nuclei were stained blue with DAPI; HDAC6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HDAC6 expression in HAP-1 cells using HDAC6 antibody (Cat#6486, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HDAC6.
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