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KD-Validated ATG5 Rabbit pAb

#65012

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shRNA Knockdown Validated
Western blotting analysis using ATG5 antibody (Cat#65012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG5 antibody (Cat#65012, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ATG5 antibody (Cat #65012). ATG5 expression in wild-type (WT) and ATG5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG5 antibody (Cat #65012, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ATG5
  • Catalog:
    65012
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated ATG5 Rabbit pAb
Aliases ATG5; Autophagy Related 5; ASP; HAPG5; APG5L; APG5; Apoptosis-Specific Protein; Autophagy Protein 5; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae); ATG5 Autophagy Related 5 Homolog; APG5 Autophagy 5-Like; APG5-LIKE; APG5-Like; SCAR25
Background

Gene Name: ATG5

NCBI Gene Entry: 9474

UniProt Entry: Q9H1Y0

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 55 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human ATG5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATG5 antibody (Cat#65012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG5 antibody (Cat#65012, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ATG5 antibody (Cat #65012). ATG5 expression in wild-type (WT) and ATG5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG5 antibody (Cat #65012, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Western blotting analysis using ATG5 antibody (Cat#65012). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG5 antibody (Cat#65012, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ATG5 antibody (Cat #65012). ATG5 expression in wild-type (WT) and ATG5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG5 antibody (Cat #65012, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
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