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Home>Product>Primary Antibody

KD-Validated SH3GL1 Mouse mAb

#65029

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shRNA Knockdown Validated
Western blotting analysis using SH3GL1 antibody (Cat#65029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SH3GL1 antibody (Cat#65029). SH3GL1 expression in wild-type (WT) and SH3GL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using SH3GL1 antibody (Cat#65029, 1:1,000), Top panel: wild-type (WT); Bottom panal: SH3GL1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SH3GL1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of SH3GL1 expression in HepG2 cells using SH3GL1 antibody (Cat#65029, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SH3GL1.
Validation of SH3GL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SH3GL1 antibody (Cat#65029, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SH3GL1
  • Catalog：
    65029
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated SH3GL1 Mouse mAb
Aliases SH3GL1; SH3 Domain Containing GRB2 Like 1, Endophilin A2; SH3D2B; CNSA1; EEN; SH3P8; Extra Eleven-Nineteen Leukemia Fusion Gene Protein; SH3 Domain-Containing GRB2-Like Protein 1; SH3-Containing Grb-2-Like 1 Protein; Extra 11-19 Leukemia Fusion; EEN Fusion Partner Of MLL; SH3-Domain GRB2-Like 1; SH3 Domain Protein 2B; Endophilin-A2; Endophilin-2; MGC111371; SH3 Domain Containing GRB2 Like 1; SH3-Containing Protein EEN; SH3 Domain GRB2-Like 1; Fusion Partner Of MLL; Endophilin A2
Background

Gene Name: SH3GL1

NCBI Gene Entry: 6455

UniProt Entry: Q99961

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 45 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13930

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human SH3GL1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SH3GL1 antibody (Cat#65029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using SH3GL1 antibody (Cat#65029). SH3GL1 expression in wild-type (WT) and SH3GL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using SH3GL1 antibody (Cat#65029, 1:1,000), Top panel: wild-type (WT); Bottom panal: SH3GL1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SH3GL1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of SH3GL1 expression in HepG2 cells using SH3GL1 antibody (Cat#65029, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SH3GL1.
  • Validation of SH3GL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SH3GL1 antibody (Cat#65029, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SH3GL1
  • Catalog：
    65029
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using SH3GL1 antibody (Cat#65029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SH3GL1 antibody (Cat#65029). SH3GL1 expression in wild-type (WT) and SH3GL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using SH3GL1 antibody (Cat#65029, 1:1,000), Top panel: wild-type (WT); Bottom panal: SH3GL1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SH3GL1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of SH3GL1 expression in HepG2 cells using SH3GL1 antibody (Cat#65029, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SH3GL1.
Validation of SH3GL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SH3GL1 antibody (Cat#65029, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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