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Home>Product>Primary Antibody

KD-Validated PIP4K2A Mouse mAb

#65034

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shRNA Knockdown Validated
Western blotting analysis using PIP4K2A antibody (Cat#65034). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PIP4K2A antibody (Cat#65034). PIP4K2A expression in wild-type (WT) and PIP4K2A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PIP4K2A expression in HT-1080 cells using PIP4K2A antibody (Cat#65034, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PIP4K2A.
Validation of PIP4K2A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PIP4K2A antibody (Cat#65034, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIP4K2A
  • Catalog：
    65034
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated PIP4K2A Mouse mAb
Aliases PIP4K2A; Phosphatidylinositol-5-Phosphate 4-Kinase Type 2 Alpha; PIP5KIIalpha; PIP5KIIA; PIP5K2A; Phosphatidylinositol-4-Phosphate 5-Kinase, Type II, Alpha; Phosphatidylinositol 5-Phosphate 4-Kinase Type II Alpha; Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Alpha; 1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-Alpha; Diphosphoinositide Kinase 2-Alpha; PI(5)P 4-Kinase Type II Alpha; PIP4KII-Alpha; EC 2.7.1.149; PI5P4Kalpha; PIP5KIII; PI5P4KA; Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Alpha; 1-Phosphatidylinositol-4-Phosphate-5-Kinase; 1-Phosphatidylinositol-4-Phosphate Kinase; Phosphatidylinositol 5-Phosphate 4-Kinase; PtdIns(5)P-4-Kinase Isoform 2-Alpha; PtdIns(4)P-5-Kinase B Isoform; PtdIns(4)P-5-Kinase C Isoform; PIP5KII-Alpha; EC 2.7.1; PIP5K2; PIPK
Background

Gene Name: PIP4K2A

NCBI Gene Entry: 5305

UniProt Entry: P48426

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 49 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13955

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human PIP4K2A
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PIP4K2A antibody (Cat#65034). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using PIP4K2A antibody (Cat#65034). PIP4K2A expression in wild-type (WT) and PIP4K2A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PIP4K2A expression in HT-1080 cells using PIP4K2A antibody (Cat#65034, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PIP4K2A.
  • Validation of PIP4K2A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PIP4K2A antibody (Cat#65034, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIP4K2A
  • Catalog：
    65034
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PIP4K2A antibody (Cat#65034). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PIP4K2A antibody (Cat#65034). PIP4K2A expression in wild-type (WT) and PIP4K2A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIP4K2A antibody (Cat#65034, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PIP4K2A expression in HT-1080 cells using PIP4K2A antibody (Cat#65034, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PIP4K2A.
Validation of PIP4K2A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PIP4K2A antibody (Cat#65034, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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