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KD-Validated REEP2 Mouse mAb

#65174

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Knockdown Validated
Western blotting analysis using REEP2 antibody (Cat#65174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using REEP2 antibody (Cat#65174). REEP2 expression in wild-type (WT) and REEP2 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of REEP2 expression in HeLa cells using REEP2 antibody (Cat#65174, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, REEP2.
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    REEP2
  • Catalog:
    65174
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name KD-Validated REEP2 Mouse mAb
Aliases REEP2; Receptor Accessory Protein 2; SGC32445; C5orf19; SPG72; Yip2d; Receptor Expression-Enhancing Protein 2; Chromosome 5 Open Reading Frame 19; SPG72A; SPG72B
Background

Gene Name: REEP2

NCBI Gene Entry: 51308

UniProt Entry: Q9BRK0

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13990

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human REEP2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using REEP2 antibody (Cat#65174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using REEP2 antibody (Cat#65174). REEP2 expression in wild-type (WT) and REEP2 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of REEP2 expression in HeLa cells using REEP2 antibody (Cat#65174, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, REEP2.
Western blotting analysis using REEP2 antibody (Cat#65174). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using REEP2 antibody (Cat#65174). REEP2 expression in wild-type (WT) and REEP2 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with REEP2 antibody (Cat#65174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of REEP2 expression in HeLa cells using REEP2 antibody (Cat#65174, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, REEP2.
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