Western blotting analysis using CDK5RAP3 antibody (Cat#65198). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDK5RAP3 antibody (Cat#65198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CDK5RAP3 antibody (Cat#65198). CDK5RAP3 expression in wild-type (WT) and CDK5RAP3 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CDK5RAP3 antibody (Cat#65198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with CDK5RAP3 antibody (Cat#65198, 1:1,000). Nuclei were stained blue with DAPI; CDK5RAP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using CDK5RAP3 antibody (Cat#65198, 1:1,000), Top panel: wild-type (WT); Bottom panal: CDK5RAP3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CDK5RAP3 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CDK5RAP3 expression in HepG2 cells using CDK5RAP3 antibody (Cat#65198, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CDK5RAP3.
