KD-Validated SRP72 Recombinant Rabbit mAb - Product

KD-Validated SRP72 Recombinant Rabbit mAb

#65606

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using SRP72 antibody (Cat#65606). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRP72 antibody (Cat#65606, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using SRP72 antibody (Cat#65606). SRP72 expression in wild-type (WT) and SRP72 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SRP72 antibody (Cat#65606, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of SRP72 expression in HepG2 cells using SRP72 antibody (Cat#65606, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SRP72.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

SRP72
Catalog：

65606
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated SRP72 Recombinant Rabbit mAb
Aliases	SRP72; Signal Recognition Particle 72; Signal Recognition Particle 72 KDa Protein; Signal Recognition Particle Subunit SRP72; Signal Recognition Particle 72kDa; Signal Recognition Particle 72kD; Epididymis Luminal Protein 103; HEL103; BMFS1; BMFF
Background	Gene Name: SRP72 NCBI Gene Entry: 6731 UniProt Entry: O76094
Application Information	Molecular Weight: Predicted, 75 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB4490 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human SRP72
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using SRP72 antibody (Cat#65606). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRP72 antibody (Cat#65606, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SRP72 antibody (Cat#65606). SRP72 expression in wild-type (WT) and SRP72 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SRP72 antibody (Cat#65606, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SRP72 expression in HepG2 cells using SRP72 antibody (Cat#65606, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SRP72.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

SRP72
Catalog：

65606
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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