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Home>Product>Primary Antibody

KD-Validated TAF1C Recombinant Rabbit mAb

#65610

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using TAF1C antibody (Cat#65610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAF1C antibody (Cat#65610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TAF1C antibody (Cat#65610). TAF1C expression in wild-type (WT) and TAF1C shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TAF1C antibody (Cat#65610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TAF1C antibody (Cat#65610, 1:1,000). Nuclei were stained blue with DAPI; TAF1C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of TAF1C expression in HepG2 cells using TAF1C antibody (Cat#65610, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TAF1C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TAF1C
  • Catalog：
    65610
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated TAF1C Recombinant Rabbit mAb
Aliases TAF1C; TATA-Box Binding Protein Associated Factor, RNA Polymerase I Subunit C; TAFI110; MGC:39976; TAFI95; SL1; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kDa; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, C, 110kD; TATA Box-Binding Protein-Associated Factor RNA Polymerase I Subunit C; RNA Polymerase I-Specific TBP-Associated Factor 110 KDa; Transcription Initiation Factor SL1/TIF-IB Subunit C; TATA Box-Binding Protein-Associated Factor 1C; TBP-Associated Factor 1C; TATA-Box Binding Protein Associated Factor, RNA Polymerase I, C; Transcription Factor SL1; SL1, 110kD Subunit
Background

Gene Name: TAF1C

NCBI Gene Entry: 9013

UniProt Entry: Q15572

Application Information

Molecular Weight: Predicted, 95 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4510

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human TAF1C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TAF1C antibody (Cat#65610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAF1C antibody (Cat#65610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using TAF1C antibody (Cat#65610). TAF1C expression in wild-type (WT) and TAF1C shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TAF1C antibody (Cat#65610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with TAF1C antibody (Cat#65610, 1:1,000). Nuclei were stained blue with DAPI; TAF1C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of TAF1C expression in HepG2 cells using TAF1C antibody (Cat#65610, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TAF1C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TAF1C
  • Catalog：
    65610
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using TAF1C antibody (Cat#65610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAF1C antibody (Cat#65610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TAF1C antibody (Cat#65610). TAF1C expression in wild-type (WT) and TAF1C shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TAF1C antibody (Cat#65610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TAF1C antibody (Cat#65610, 1:1,000). Nuclei were stained blue with DAPI; TAF1C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of TAF1C expression in HepG2 cells using TAF1C antibody (Cat#65610, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TAF1C.
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