KD-Validated MRPS18B Recombinant Rabbit mAb - Product

KD-Validated MRPS18B Recombinant Rabbit mAb

#65651

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using MRPS18B antibody (Cat#65651). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRPS18B antibody (Cat#65651, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using MRPS18B antibody (Cat#65651). MRPS18B expression in wild-type (WT) and MRPS18B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MRPS18B antibody (Cat#65651, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of MRPS18B expression in HT-1080 cells using MRPS18B antibody (Cat#65651, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MRPS18B.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MRPS18B
Catalog：

65651
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated MRPS18B Recombinant Rabbit mAb
Aliases	MRPS18B; Mitochondrial Ribosomal Protein S18B; C6orf14; MRPS18-2; HSPC183; PTD017; MS40; 28S Ribosomal Protein S18-2, Mitochondrial; 28S Ribosomal Protein S18b, Mitochondrial; Small Ribosomal Subunit Protein BS18b; Small Ribosomal Subunit Protein MS40; MRP-S18-2; MRP-S18-B; Mrps18-B; S18mt-B; Mitochondrial Small Ribosomal Subunit Protein BS18m-B; Mitochondrial Small Ribosomal Subunit Protein BS18b; Mitochondrial Small Ribosomal Subunit Protein MS40; Mitochondrial Ribosomal Protein S18-2; HumanS18a; S18amt
Background	Gene Name: MRPS18B NCBI Gene Entry: 28973 UniProt Entry: Q9Y676
Application Information	Molecular Weight: Predicted, 29 kDa; observed, 29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB4705 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human MRPS18B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using MRPS18B antibody (Cat#65651). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRPS18B antibody (Cat#65651, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MRPS18B antibody (Cat#65651). MRPS18B expression in wild-type (WT) and MRPS18B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MRPS18B antibody (Cat#65651, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MRPS18B expression in HT-1080 cells using MRPS18B antibody (Cat#65651, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MRPS18B.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MRPS18B
Catalog：

65651
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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