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Home>Product>Primary Antibody

KD-Validated ILVBL Mouse mAb

#65852

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shRNA Knockdown Validated
Western blotting analysis using ILVBL antibody (Cat#65852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ILVBL antibody (Cat#65852). ILVBL expression in wild-type (WT) and ILVBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using ILVBL antibody (Cat#65852, 1:1,000), Top panel: wild-type (WT); Bottom panal: ILVBL shRNA knockdown (KD). Nuclei were stained blue with DAPI; ILVBL was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ILVBL expression in HepG2 cells using ILVBL antibody (Cat#65852, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ILVBL.
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Unit price：
Quantity：
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  • Gene Symbol：
    HACL2
  • Catalog：
    65852
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated ILVBL Mouse mAb
Aliases HACL2; ILVBL; IlvB Acetolactate Synthase Like; AHAS; HACL1L; 209L8; ILV2H; IlvB (Bacterial Acetolactate Synthase)-Like; Acetolactate Synthase-Like Protein; 2-Hydroxyacyl-CoA Lyase 1 Like; Acetolactate Synthase Homolog; 2-Hydroxyacyl-CoA Lyase 2; IlvB-Like Protein; FLJ39061; MGC19535; MGC1269; EC 4.1.2.-; EC 2.2.1
Background

Gene Name: HACL2

NCBI Gene Entry: 10994

UniProt Entry: A1L0T0

Application Information

Molecular Weight: Predicted, 68 kDa; observed, 62 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5180

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ILVBL
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ILVBL antibody (Cat#65852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ILVBL antibody (Cat#65852). ILVBL expression in wild-type (WT) and ILVBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using ILVBL antibody (Cat#65852, 1:1,000), Top panel: wild-type (WT); Bottom panal: ILVBL shRNA knockdown (KD). Nuclei were stained blue with DAPI; ILVBL was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ILVBL expression in HepG2 cells using ILVBL antibody (Cat#65852, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ILVBL.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HACL2
  • Catalog：
    65852
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using ILVBL antibody (Cat#65852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ILVBL antibody (Cat#65852). ILVBL expression in wild-type (WT) and ILVBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using ILVBL antibody (Cat#65852, 1:1,000), Top panel: wild-type (WT); Bottom panal: ILVBL shRNA knockdown (KD). Nuclei were stained blue with DAPI; ILVBL was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ILVBL expression in HepG2 cells using ILVBL antibody (Cat#65852, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ILVBL.
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