Western blotting analysis using nudix hydrolase 21 antibody (Cat#65920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with nudix hydrolase 21 antibody (Cat#65920, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using nudix hydrolase 21 antibody (Cat#65920). Nudix hydrolase 21 expression in wild-type (WT) and nudix hydrolase 21 (NUDT21) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with nudix hydrolase 21 antibody (Cat#65920, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Nudix hydrolase 21 expression in HAP-1 cells using Nudix hydrolase 21 antibody (Cat#65920, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Nudix hydrolase 21.
Validation of Nudix hydrolase 21 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Nudix hydrolase 21 antibody (Cat#65920, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
