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Home>Product>Primary Antibody

KD-Validated M6PR Mouse mAb

#65936

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shRNA Knockdown Validated
Western blotting analysis using M6PR antibody (Cat#65936). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with M6PR antibody (Cat#65936, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using M6PR antibody (Cat#65936). M6PR expression in wild-type (WT) and M6PR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with M6PR antibody (Cat#65936, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of M6PR expression in HAP-1 cells using M6PR antibody (Cat#65936, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, M6PR.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
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  • Gene Symbol：
    M6PR
  • Catalog：
    65936
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated M6PR Mouse mAb
Aliases M6PR; Mannose-6-Phosphate Receptor, Cation Dependent; CD-MPR; CD-M6PR; Cation-Dependent Mannose-6-Phosphate Receptor; CD Man-6-P Receptor; MPR 46; MPR46; Mannose-6-Phosphate Receptor (Cation Dependent); 46-KDa Mannose 6-Phosphate Receptor; 46 KDa Mannose 6-Phosphate Receptor; Small Mannose 6-Phosphate Receptor; Mr 46,000 Man6PR; MPR-46; SMPR; MPRD
Background

Gene Name: M6PR

NCBI Gene Entry: 4074

UniProt Entry: P20645

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 37 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5915

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human M6PR
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using M6PR antibody (Cat#65936). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with M6PR antibody (Cat#65936, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using M6PR antibody (Cat#65936). M6PR expression in wild-type (WT) and M6PR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with M6PR antibody (Cat#65936, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of M6PR expression in HAP-1 cells using M6PR antibody (Cat#65936, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, M6PR.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    M6PR
  • Catalog：
    65936
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using M6PR antibody (Cat#65936). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with M6PR antibody (Cat#65936, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using M6PR antibody (Cat#65936). M6PR expression in wild-type (WT) and M6PR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with M6PR antibody (Cat#65936, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of M6PR expression in HAP-1 cells using M6PR antibody (Cat#65936, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, M6PR.
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