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Home>Product>Primary Antibody

KD-Validated PDK2 Mouse mAb

#66118

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Knockdown Validated
Western blotting analysis using PDK2 antibody (Cat#66118). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDK2 antibody (Cat#66118, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PDK2 antibody (Cat#66118). PDK2 expression in wild type (WT) and PDK2 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDK2 antibody (Cat#66118, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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  • Gene Symbol：
    PDK2
  • Catalog：
    66118
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated PDK2 Mouse mAb
Aliases PDK2; Pyruvate Dehydrogenase Kinase 2; PDHK2; [Pyruvate Dehydrogenase (Acetyl-Transferring)] Kinase Isozyme 2, Mitochondrial; Pyruvate Dehydrogenase Kinase, Isoenzyme 2; Pyruvate Dehydrogenase Kinase, Isozyme 2; PDH Kinase 2; EC 2.7.11.2; PDKII; Pyruvate Dehydrogenase, Lipoamide, Kinase Isozyme 2, Mitochondrial; Pyruvate Dehydrogenase Kinase Isoform 2; EC 2.7.11
Background

Gene Name: PDK2

NCBI Gene Entry: 5164

UniProt Entry: Q15119

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB7155

Species Reactivity: Human, mouse, rat

Applications Tested: Western Blotting (WB)
Immunogen Recombinant protein of human PDK2
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PDK2 antibody (Cat#66118). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDK2 antibody (Cat#66118, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using PDK2 antibody (Cat#66118). PDK2 expression in wild type (WT) and PDK2 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDK2 antibody (Cat#66118, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDK2
  • Catalog：
    66118
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PDK2 antibody (Cat#66118). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDK2 antibody (Cat#66118, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PDK2 antibody (Cat#66118). PDK2 expression in wild type (WT) and PDK2 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDK2 antibody (Cat#66118, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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