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Home>Product>Primary Antibody

ITGAX Mouse mAb

#6663

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Western blotting analysis using ITGAX antibody (Cat#6663). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITGAX antibody (Cat#6663, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using ITGAX antibody (Cat#6663, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of THP-1 cells with ITGAX antibody (Cat#6663, 1:1,000). Nuclei were stained blue with DAPI; ITGAX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    ITGAX
  • Catalog：
    6663
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name ITGAX Mouse mAb
Aliases ITGAX; Integrin Subunit Alpha X; CD11c; Integrin, Alpha X (Complement Component 3 Receptor 4 Subunit); Integrin, Alpha X (Antigen CD11C (P150), Alpha Polypeptide); Leukocyte Adhesion Glycoprotein P150,95 Alpha Chain; Complement Component 3 Receptor 4 Subunit; Leukocyte Adhesion Receptor P150,95; CD11 Antigen-Like Family Member C; Integrin Alpha-X; Leukocyte Surface Antigen P150,95, Alpha Subunit; Myeloid Membrane Antigen, Alpha Subunit; P150 95 Integrin Alpha Chain; Leu M5, Alpha Subunit; Integrin Alpha X; CD11c Antigen; Leu M5; SLEB6; CD11C
Background

Gene Name: ITGAX

NCBI Gene Entry: 3687

UniProt Entry: P20702

Application Information

Molecular Weight: Predicted, 128 kDa; observed, 150 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB250

Species Reactivity: Human

Applications Tested: Western Blotting (WB), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human ITGAX
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using ITGAX antibody (Cat#6663). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITGAX antibody (Cat#6663, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using ITGAX antibody (Cat#6663, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of THP-1 cells with ITGAX antibody (Cat#6663, 1:1,000). Nuclei were stained blue with DAPI; ITGAX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ITGAX
  • Catalog：
    6663
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ITGAX antibody (Cat#6663). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ITGAX antibody (Cat#6663, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using ITGAX antibody (Cat#6663, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of THP-1 cells with ITGAX antibody (Cat#6663, 1:1,000). Nuclei were stained blue with DAPI; ITGAX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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