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KD-Validated XPNPEP1 Mouse mAb

#66816

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shRNA Knockdown Validated
Western blotting analysis using XPNPEP1 antibody (Cat#66816). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XPNPEP1 antibody (Cat#66816, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using XPNPEP1 antibody (Cat#66816). XPNPEP1 expression in wild type (WT) and XPNPEP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with XPNPEP1 antibody (Cat#66816, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    XPNPEP1
  • Catalog:
    66816
  • Application:
    WB
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated XPNPEP1 Mouse mAb
Aliases XPNPEP1; X-Prolyl Aminopeptidase 1; Xaa-Pro Aminopeptidase 1; XPNPEPL1; XPNPEPL; X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble; X-Prolyl Aminopeptidase 1, Soluble; Aminoacylproline Aminopeptidase; Cytosolic Aminopeptidase P; Soluble Aminopeptidase P; X-Pro Aminopeptidase 1; EC 3.4.11.9; XPNPEP; X-Prolyl Aminopeptidase (Aminopeptidase P)-Like; Aminopeptidase P, Cytosolic; APP1; SAMP; SAmp
Background

Gene Name: XPNPEP1

NCBI Gene Entry: 7511

UniProt Entry: Q9NQW7

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 70-75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1835

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human XPNPEP1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using XPNPEP1 antibody (Cat#66816). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XPNPEP1 antibody (Cat#66816, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using XPNPEP1 antibody (Cat#66816). XPNPEP1 expression in wild type (WT) and XPNPEP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with XPNPEP1 antibody (Cat#66816, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using XPNPEP1 antibody (Cat#66816). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XPNPEP1 antibody (Cat#66816, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using XPNPEP1 antibody (Cat#66816). XPNPEP1 expression in wild type (WT) and XPNPEP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with XPNPEP1 antibody (Cat#66816, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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