Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated PIN1 Recombinant Rabbit mAb

#66914

Click to copy product information

Return to List

Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PIN1 antibody (Cat#66914). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIN1 antibody (Cat#66914, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PIN1 antibody (Cat#66914). PIN1 expression in wild type (WT) and PIN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with PIN1 antibody (Cat#66914, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PIN1 antibody (Cat#66914, 1:1,000). Nuclei were stained blue with DAPI; PIN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIN1
  • Catalog：
    66914
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated PIN1 Recombinant Rabbit mAb
Aliases PIN1; Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 1; Dod; Protein (Peptidyl-Prolyl Cis/Trans Isomerase) NIMA-Interacting 1; Peptidyl-Prolyl Cis-Trans Isomerase NIMA-Interacting 1; Protein Interacting With Never In Mitosis A1; Parvulin PIN1; Rotamase Pin1; PPIase Pin1; EC 5.2.1.8; Peptidyl-Prolyl Cis-Trans Isomerase Pin1; UBL5
Background

Gene Name: PIN1

NCBI Gene Entry: 5300

UniProt Entry: Q13526

Application Information

Molecular Weight: Predicted, 18 kDa; observed, 18 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1315

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PIN1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PIN1 antibody (Cat#66914). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIN1 antibody (Cat#66914, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using PIN1 antibody (Cat#66914). PIN1 expression in wild type (WT) and PIN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with PIN1 antibody (Cat#66914, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with PIN1 antibody (Cat#66914, 1:1,000). Nuclei were stained blue with DAPI; PIN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIN1
  • Catalog：
    66914
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PIN1 antibody (Cat#66914). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIN1 antibody (Cat#66914, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PIN1 antibody (Cat#66914). PIN1 expression in wild type (WT) and PIN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with PIN1 antibody (Cat#66914, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with PIN1 antibody (Cat#66914, 1:1,000). Nuclei were stained blue with DAPI; PIN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.