Western blotting analysis using FLOT1 antibody (Cat#66969). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLOT1 antibody (Cat#66969, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FLOT1 antibody (Cat#66969). FLOT1 expression in wild-type (WT) and FLOT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FLOT1 antibody (Cat#66969, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using FLOT1 antibody (Cat#66969). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLOT1 antibody (Cat#66969, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FLOT1 antibody (Cat#66969). FLOT1 expression in wild-type (WT) and FLOT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FLOT1 antibody (Cat#66969, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FLOT1 antibody (Cat#66969). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLOT1 antibody (Cat#66969, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FLOT1 antibody (Cat#66969). FLOT1 expression in wild-type (WT) and FLOT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FLOT1 antibody (Cat#66969, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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