Western blotting analysis using EPHB2 antibody (Cat#67015, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using EPHB2 antibody (Cat#67015, 1:1,000). EPHB2 expression in wild-type (WT) and EPHB2 shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Immunocytochemical staining of MCF7 cells using EPHB2 antibody (Cat#67015, 1:200). Top panel: wild type (WT); Bottom panel: EPHB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was atained green with Alexs Fluor 488; EPHB2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human EPHB2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000 Immunocytochemistry (ICC): 1:200
Note
This product is for research use only.
Data
Western blotting analysis using EPHB2 antibody (Cat#67015, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using EPHB2 antibody (Cat#67015, 1:1,000). EPHB2 expression in wild-type (WT) and EPHB2 shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Immunocytochemical staining of MCF7 cells using EPHB2 antibody (Cat#67015, 1:200). Top panel: wild type (WT); Bottom panel: EPHB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was atained green with Alexs Fluor 488; EPHB2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Western blotting analysis using EPHB2 antibody (Cat#67015, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using EPHB2 antibody (Cat#67015, 1:1,000). EPHB2 expression in wild-type (WT) and EPHB2 shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Immunocytochemical staining of MCF7 cells using EPHB2 antibody (Cat#67015, 1:200). Top panel: wild type (WT); Bottom panel: EPHB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; actin was atained green with Alexs Fluor 488; EPHB2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
