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KD-Validated PPP2CA Recombinant Rabbit mAb

#67028

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PPP2CA antibody (Cat#67028, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PPP2CA antibody (Cat#67028, 1:1,000). PPP2CA expression in wild-type (WT) and PPP2CA shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PPP2CA antibody (Cat#67028). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2CA antibody (Cat#67028, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
Immunocytochemical staining of HeLa cells using PPP2CA antibody (Cat#67028, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; PPP2CA was stained magenta with Alexa Flour® 647.
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  • Gene Symbol:
    PPP2CA
  • Catalog:
    67028
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R, Mk, D, C
Information
Product Name KD-Validated PPP2CA Recombinant Rabbit mAb
Aliases PPP2CA; Protein Phosphatase 2 Catalytic Subunit Alpha; Serine/Threonine-Protein Phosphatase 2A Catalytic Subunit Alpha Isoform; Protein Phosphatase 2, Catalytic Subunit, Alpha Isozyme; Replication Protein C; EC 3.1.3.16; PP2A-Alpha; PP2Calpha; PP2AC; RP-C; Protein Phosphatase 2 (Formerly 2A), Catalytic Subunit, Alpha Isoform; Protein Phosphatase 2A Catalytic Subunit, Alpha Isoform; PP2CALPHA; NEDLBA; PP2Ac; PP2CA; HJS3
Background

Gene Name: PPP2CA

NCBI Gene Entry: 5515

UniProt Entry: P67775

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 38,36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2460

Species Reactivity: Human, mouse, rat, monkey, dog, cow

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human PPP2CA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Immunocytochemistry (ICC): 1:100
Note This product is for research use only.
Data
  • Western blotting analysis using PPP2CA antibody (Cat#67028, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using PPP2CA antibody (Cat#67028, 1:1,000). PPP2CA expression in wild-type (WT) and PPP2CA shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
  • Western blotting analysis using PPP2CA antibody (Cat#67028). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2CA antibody (Cat#67028, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
  • Immunocytochemical staining of HeLa cells using PPP2CA antibody (Cat#67028, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; PPP2CA was stained magenta with Alexa Flour® 647.
Western blotting analysis using PPP2CA antibody (Cat#67028, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PPP2CA antibody (Cat#67028, 1:1,000). PPP2CA expression in wild-type (WT) and PPP2CA shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PPP2CA antibody (Cat#67028). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2CA antibody (Cat#67028, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
Immunocytochemical staining of HeLa cells using PPP2CA antibody (Cat#67028, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; PPP2CA was stained magenta with Alexa Flour® 647.
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