Western blotting analysis using SIRT2 antibody (Cat#67044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIRT2 antibody (Cat#67044, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SIRT2 antibody (Cat#67044). SIRT2 expression in wild-type (WT) and SIRT2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SIRT2 antibody (Cat#67044, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
SIRT2; Sirtuin 2; SIR2L; NAD-Dependent Protein Defatty-Acylase Sirtuin-2; NAD-Dependent Protein Deacetylase Sirtuin-2; Regulatory Protein SIR2 Homolog 2; SIR2-Like Protein 2; SIR2L2; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 2 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 2; NAD-Dependent Deacetylase Sirtuin-2; Silent Information Regulator 2; Sir2-Related Protein Type 2; Sirtuin Type 2; EC 2.3.1.286; EC 2.3.1.-; SIR2
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using SIRT2 antibody (Cat#67044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIRT2 antibody (Cat#67044, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SIRT2 antibody (Cat#67044). SIRT2 expression in wild-type (WT) and SIRT2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SIRT2 antibody (Cat#67044, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SIRT2 antibody (Cat#67044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SIRT2 antibody (Cat#67044, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SIRT2 antibody (Cat#67044). SIRT2 expression in wild-type (WT) and SIRT2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SIRT2 antibody (Cat#67044, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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