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Home>Product>Primary Antibody

HMCES Recombinant Mouse mAb

#6719

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Recombinant Antibody
Western blotting analysis using HMCES antibody (Cat#6719). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HMCES antibody (Cat#6719, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with HMCES antibody (Cat#6719, 1:1,000). Nuclei were stained blue with DAPI; HMCES was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HMCES
  • Catalog：
    6719
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name HMCES Recombinant Mouse mAb
Aliases HMCES; 5-Hydroxymethylcytosine Binding, ES Cell Specific; SRAPD1; DC12; Embryonic Stem Cell-Specific 5-Hydroxymethylcytosine-Binding Protein; C3orf37; 5-Hydroxymethylcytosine (HmC) Binding, ES Cell-Specific; SOS Response Associated Peptidase Domain Containing 1; ES Cell-Specific 5hmC-Binding Protein; Abasic Site Processing Protein HMCES; SRAP Domain-Containing Protein 1; Peptidase HMCES; Chromosome 3 Open Reading Frame 37; Putative Endonuclease HMCES; Putative Peptidase SRAPD1; UPF0361 Protein C3orf37; EC 4.-.-.-; EC 3.4.-.-
Background

Gene Name: HMCES

NCBI Gene Entry: 56941

UniProt Entry: Q96FZ2

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 36 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB525

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HMCES
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HMCES antibody (Cat#6719). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HMCES antibody (Cat#6719, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells with HMCES antibody (Cat#6719, 1:1,000). Nuclei were stained blue with DAPI; HMCES was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HMCES
  • Catalog：
    6719
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using HMCES antibody (Cat#6719). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HMCES antibody (Cat#6719, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells with HMCES antibody (Cat#6719, 1:1,000). Nuclei were stained blue with DAPI; HMCES was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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