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PTPN7 Recombinant Mouse mAb

#6720

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Recombinant Antibody
Western blotting analysis using PTPN7 antibody (Cat#6720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPN7 antibody (Cat#6720, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Jurkat cells with PTPN7 antibody (Cat#6720, 1:1,000). Nuclei were stained blue with DAPI; PTPN7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PTPN7
  • Catalog:
    6720
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name PTPN7 Recombinant Mouse mAb
Aliases PTPN7; Protein Tyrosine Phosphatase Non-Receptor Type 7; HEPTP; LC-PTP; Tyrosine-Protein Phosphatase Non-Receptor Type 7; Hematopoietic Protein-Tyrosine Phosphatase; Protein-Tyrosine Phosphatase LC-PTP; EC 3.1.3.48; Protein-Tyrosine Phosphatase, Nonreceptor-Type, Stress Induced; Dual Specificity Phosphatase 1; BPTP-4; PTPNI; LPTP
Background

Gene Name: PTPN7

NCBI Gene Entry: 5778

UniProt Entry: P35236

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 37-40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB530

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human PTPN7
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PTPN7 antibody (Cat#6720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPN7 antibody (Cat#6720, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Jurkat cells with PTPN7 antibody (Cat#6720, 1:1,000). Nuclei were stained blue with DAPI; PTPN7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using PTPN7 antibody (Cat#6720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPN7 antibody (Cat#6720, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Jurkat cells with PTPN7 antibody (Cat#6720, 1:1,000). Nuclei were stained blue with DAPI; PTPN7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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