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Home>Product>Primary Antibody

SMARCA4 Mouse mAb

#6757

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Western blotting analysis using SMARCA4 antibody (Cat#6757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA4 antibody (Cat#6757, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with SMARCA4 antibody (Cat#6757, 1:1,000). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCA4
  • Catalog：
    6757
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SMARCA4 Mouse mAb
Aliases SMARCA4; SWI/SNF Related BAF Chromatin Remodeling Complex Subunit ATPase 4; BRG1; Mitotic Growth And Transcription Activator; SNF2-BETA; HSNF2b; BAF190; SNF2LB; SNF2L4; SNF2; SWI2; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4; Global Transcription Activator Homologous Sequence; ATP-Dependent Helicase SMARCA4; Sucrose Nonfermenting-Like 4; Transcription Activator BRG1; BRG1-Associated Factor 190A; Protein Brahma Homolog 1; BRM/SWI2-Related Gene 1; Homeotic Gene Regulator; Brahma Protein-Like 1; Nuclear Protein GRB1; Protein BRG-1; SNF2-Like 4; FLJ39786; BAF190A; EC 3.6.4.-; SNF2-Beta; EC 3.6.1; OTSC12; MRD16; RTPS2; SNF2B; CSS4
Background

Gene Name: SMARCA4

NCBI Gene Entry: 6597

UniProt Entry: P51532

Application Information

Molecular Weight: Predicted, 185 kDa; observed, 225 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB750

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human SMARCA4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMARCA4 antibody (Cat#6757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA4 antibody (Cat#6757, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with SMARCA4 antibody (Cat#6757, 1:1,000). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCA4
  • Catalog：
    6757
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SMARCA4 antibody (Cat#6757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA4 antibody (Cat#6757, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with SMARCA4 antibody (Cat#6757, 1:1,000). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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