Western blotting analysis using VHL antibody (Cat#6774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VHL antibody (Cat#6774, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
VHL; Von Hippel-Lindau Tumor Suppressor; VHL1; Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase; Von Hippel-Lindau Disease Tumor Suppressor; Protein G7; PVHL; Von Hippel-Lindau Syndrome; Elongin Binding Protein; HRCA1; RCA1
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using VHL antibody (Cat#6774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VHL antibody (Cat#6774, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using VHL antibody (Cat#6774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VHL antibody (Cat#6774, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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