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SMARCC1 Mouse Oligoclonal Antibody

#6809

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Western blotting analysis using SMARCC1 antibody (Cat#6809). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCC1 antibody (Cat#6809, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with SMARCC1 antibody (Cat#6809, 1:1,000). Nuclei were stained blue with DAPI; SMARCC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SMARCC1
  • Catalog:
    6809
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name SMARCC1 Mouse Oligoclonal Antibody
Aliases SMARCC1; SWI/SNF Related BAF Chromatin Remodeling Complex Subunit C1; BAF155; CRACC1; SRG3; Rsc8; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin Subfamily C Member 1; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily C Member 1; SWI/SNF Complex Subunit SMARCC1; SWI/SNF Complex 155 KDa Subunit; BRG1-Associated Factor 155; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily C, Member 1; Mammalian Chromatin Remodeling Complex BRG1-Associated Factor 155; Chromatin Remodeling Complex BAF155 Subunit; HYC5; SWI3
Background

Gene Name: SMARCC1

NCBI Gene Entry: 6599

UniProt Entry: Q92922

Application Information

Molecular Weight: Predicted, 123 kDa; observed, 155 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human SMARCC1
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:4,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMARCC1 antibody (Cat#6809). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCC1 antibody (Cat#6809, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with SMARCC1 antibody (Cat#6809, 1:1,000). Nuclei were stained blue with DAPI; SMARCC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using SMARCC1 antibody (Cat#6809). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCC1 antibody (Cat#6809, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with SMARCC1 antibody (Cat#6809, 1:1,000). Nuclei were stained blue with DAPI; SMARCC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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